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Biosensors: CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics (Adv. Mater. 51/2019)
Author(s) -
Bruch Richard,
Baaske Julia,
Chatelle Claire,
Meirich Mailin,
Madlener Sibylle,
Weber Wilfried,
Dincer Can,
Urban Gerald Anton
Publication year - 2019
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201970365
Subject(s) - biosensor , nucleic acid , crispr , microfluidics , nanotechnology , materials science , combinatorial chemistry , chemistry , biochemistry , gene
In article number 1905311, Can Dincer and co‐workers introduce the first CRISPR‐powered electrochemical microfluidic biosensor (CRISPR‐Biosensor), allowing nucleic‐acid‐amplification‐free detection of microRNAs in isolated serum samples. Upon recognition of its target, the activated Cas13a cleaves reporter RNAs in its surroundings, resulting in a decrease of the measured electrochemical signals. The CRISPR‐Biosensor offers a low‐cost, easily scalable, specific, and target amplification‐free (i.e., rapid and easy‐to‐use) tool for on‐site nucleic acid diagnostics.