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Gene Delivery: Inhaled Nanoformulated mRNA Polyplexes for Protein Production in Lung Epithelium (Adv. Mater. 8/2019)
Author(s) -
Patel Asha Kumari,
Kaczmarek James C.,
Bose Suman,
Kauffman Kevin J.,
Mir Faryal,
Heartlein Michael W.,
DeRosa Frank,
Langer Robert,
Anderson Daniel G.
Publication year - 2019
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201970053
Subject(s) - messenger rna , epithelium , materials science , lung , rna , microbiology and biotechnology , reporter gene , biology , biochemistry , gene , gene expression , medicine , pathology
In vitro transcribed messenger RNA is nebulized using poly(beta amino esters) for inhalable delivery to the lung as described by Daniel G. Anderson and co‐workers in article number 1805116 . Hyperbranched polymers are synthesized, which form stable mRNA‐polyplexes that distribute uniformly throughout the lungs of mice and are translated to reporter protein in the lung epithelium.