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A Surface‐Tailoring Method for Rapid Non‐Thermosensitive Cell‐Sheet Engineering via Functional Polymer Coatings
Author(s) -
Baek Jieung,
Cho Younghak,
Park HyunJi,
Choi Goro,
Lee Jong Seung,
Lee Minseok,
Yu Seung Jung,
Cho SeungWoo,
Lee Eunjung,
Im Sung Gap
Publication year - 2020
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201907225
Subject(s) - materials science , tissue engineering , cell , substrate (aquarium) , surface engineering , extracellular matrix , nanotechnology , transplantation , polymer , biomedical engineering , biophysics , composite material , microbiology and biotechnology , chemistry , medicine , biochemistry , oceanography , surgery , biology , geology
Cell sheet engineering, a technique utilizing a monolayer cell sheet, has recently emerged as a promising technology for scaffold‐free tissue engineering. In contrast to conventional tissue‐engineering approaches, the cell sheet technology allows cell harvest as a continuous cell sheet with intact extracellular matrix proteins and cell–cell junction, which facilitates cell transplantation without any other artificial biomaterials. A facile, non‐thermoresponsive method is demonstrated for a rapid but highly reliable platform for cell‐sheet engineering. The developed method exploits the precise modulation of cell–substrate interactions by controlling the surface energy of the substrate via a series of functional polymer coatings to enable prompt cell sheet harvesting within 100 s. The engineered surface can trigger an intrinsic cellular response upon the depletion of divalent cations, leading to spontaneous cell sheet detachment under physiological conditions (pH 7.4 and 37 °C) in a non‐thermoresponsive manner. Additionally, the therapeutic potential of the cell sheet is successfully demonstrated by the transplantation of multilayered cell sheets into mouse models of diabetic wounds and ischemia. These findings highlight the ability of the developed surface for non‐thermoresponsive cell sheet engineering to serve as a robust platform for regenerative medicine and provide significant breakthroughs in cell sheet technology.