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Real‐Time Quantification of Cell Internalization Kinetics by Functionalized Bioluminescent Nanoprobes
Author(s) -
Wu Di,
Yang Yilong,
Xu Pengcheng,
Xu Duo,
Liu Yang,
Castillo Roxanne,
Yan Ran,
Ren Jie,
Zhou George,
Liu Chaoyong,
Qin Meng,
Du Juanjuan,
Hou Lihua,
Chen Irvin,
Kang Chunsheng,
Jin Lihua,
Wen Jing,
Chen Wei,
Lu Yunfeng
Publication year - 2019
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201902469
Subject(s) - kinetics , internalization , rational design , biomolecule , luciferase , nanotechnology , fluorescence , nanocapsules , biophysics , multiplex , cell , materials science , chemistry , biology , biochemistry , nanoparticle , bioinformatics , transfection , physics , quantum mechanics , gene
Cells transport mass dynamically, crossing cell membranes to maintain metabolism and systemic homeostasis, through which biomolecules are also delivered to cells for gene editing, cell reprograming, therapy, and other purposes. Quantifying the translocation kinetics is fundamentally and clinically essential, but remains limited by fluorescence‐based technologies, which are semi‐quantitative and only provide kinetics information at cellular level or in discrete time. Herein, a real‐time method of quantifying cell internalization kinetics is reported using functionalized firefly‐luciferase nanocapsules as the probe. This quantitative assay will facilitate the rational design of delivery vectors and enable high‐throughput screening of peptides and other functional molecules, constituting an effective tool for broad applications, including drug development and cancer therapy.