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Cancer Cell Membrane Camouflaged Nanoprobe for Catalytic Ratiometric Photoacoustic Imaging of MicroRNA in Living Mice
Author(s) -
Zhang Kai,
Meng Xiangdan,
Yang Zhou,
Cao Yu,
Cheng Yaru,
Wang Dongdong,
Lu Huiting,
Shi Zhuojie,
Dong Haifeng,
Zhang Xueji
Publication year - 2019
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201807888
Subject(s) - nanoprobe , detection limit , materials science , glutathione , dna , fluorophore , biophysics , liposome , photoacoustic imaging in biomedicine , mesoporous silica , rational design , membrane , nanotechnology , nanoparticle , catalysis , chemistry , mesoporous material , fluorescence , biochemistry , biology , chromatography , physics , quantum mechanics , optics , enzyme
Herein, a cancer cell (MCF‐7 cell) membrane‐encapsulated dendritic mesoporous silica nanoparticle simultaneously functionalized with DNA‐photoacoustic (DNA‐PA) probes and glutathione (GSH)‐responsive DNA fuel strands for PA imaging of tumor‐related miRNA in living mice with signal amplification ability is developed. It is demonstrated that one target miRNA can trigger disassembly of multiple PA fluorophore probes from the quencher with the aid of GSH‐responsive DNA fuel strands via the entropy‐driven process, resulting remarkable amplified change of PA signal ratio. Using oncogenic miRNA‐21 as a model, a linear relationship between miRNA‐21 concentrations and PA ratio in a dynamic range from 10 × 10 −12 m to 100 × 10 −9 m and a limit of detection down to 11.69 × 10 −12 m are established. The accurate PA signal observation related to miRNA‐21s in the tumor area in living mice is demonstrated, and the PA signal ratio increases significantly via the injection of miRNA‐21. It is anticipated that the catalytic ratiometric PA imaging system can be applied to an array of molecular detection in living system by rational detection probe design.