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Programming Niche Accessibility and In Vitro Stemness with Intercellular DNA Reactions
Author(s) -
Li Xiaojiao,
Xie Xiaodong,
Ma Zhiwei,
Li Qian,
Liu Lin,
Hu Xingjie,
Liu Chang,
Li Bin,
Wang Hui,
Chen Nan,
Fan Chunhai,
Song Haiyun
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201804861
Subject(s) - microbiology and biotechnology , stem cell , regenerative medicine , biology , extracellular matrix , stem cell niche , niche , induced pluripotent stem cell , morphogen , embryonic stem cell , progenitor cell , genetics , biochemistry , gene
Stem cells generally exist in low abundance and tend to lose stemness in the absence of self‐renewal signals. While extracellular‐matrix‐mimicking techniques have been developed to support stem cell proliferation, the lack of niche cells in these synthetic systems often hampers continuous stem cell expansion and maintenance of pluripotency, which are indispensable for regenerative medicine. Here, an intercellular DNA‐reaction‐programmed ESPN ( e xpansion of s tem cells with p airing n iches) strategy is developed for 3D culture of mammary stem cells (MaSCs). Boolean logic operations are implemented to confer DNA‐programmed mechanical signaling and genetically engineered morphogen signaling by niche cells, resulting in sustained expansion of MaSCs in vitro. The creation of stem cell niches improves the proliferation of pluripotent cells by four times during one‐week culture. This method thus provides a novel approach for logical regulation of stemness and proliferation of stem cells for biomedicine.