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Automated Buildup of Biomimetic Films in Cell Culture Microplates for High‐Throughput Screening of Cellular Behaviors
Author(s) -
Machillot Paul,
Quintal Catarina,
Dalonneau Fabien,
Hermant Loic,
Monnot Pauline,
Matthews Kelsey,
Fitzpatrick Vincent,
Liu Jie,
PignotPaintrand Isabelle,
Picart Catherine
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201801097
Subject(s) - materials science , nanotechnology , regenerative medicine , layer by layer , adhesion , cell adhesion , throughput , high throughput screening , layer (electronics) , thin film , cell , chemistry , computer science , bioinformatics , biology , biochemistry , telecommunications , composite material , wireless
An automatic method is established for layer‐by‐layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid‐handling robot. Highly homogeneous thin films are formed at the bottom of each microwell. The LbL film‐coated microplates are compatible with common cellular assays, using microplate readers and automated microscopes. Cellular adhesion is screened on crosslinked and peptide‐functionalized LbL films and stem cell differentiation in response to increasing doses of bone morphogenetic proteins (2, 4, 7, 9). This method paves the way for future applications of LbL films in cell‐based assays for regenerative medicine and high‐throughput drug screening.