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Microfluidics‐Enabled Multimaterial Maskless Stereolithographic Bioprinting
Author(s) -
Miri Amir K.,
Nieto Daniel,
Iglesias Luis,
Goodarzi Hosseinabadi Hossein,
Maharjan Sushila,
RuizEsparza Guillermo U.,
Khoshakhlagh Parastoo,
Manbachi Amir,
Dokmeci Mehmet Remzi,
Chen Shaochen,
Shin Su Ryon,
Zhang Yu Shrike,
Khademhosseini Ali
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201800242
Subject(s) - stereolithography , biofabrication , microfluidics , materials science , nanotechnology , tissue engineering , 3d bioprinting , self healing hydrogels , biomedical engineering , fabrication , regenerative medicine , ethylene glycol , engineering , chemistry , cell , medicine , biochemistry , alternative medicine , pathology , chemical engineering , polymer chemistry , composite material
A stereolithography‐based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized by a moving stage and a microfluidic device containing four on/off pneumatic valves, is used to create 3D constructs. The novel microfluidic device is capable of fast switching between different (cell‐loaded) hydrogel bioinks, to achieve layer‐by‐layer multimaterial bioprinting. Compared to conventional stereolithography‐based bioprinters, the system provides the unique advantage of multimaterial fabrication capability at high spatial resolution. To demonstrate the multimaterial capacity of this system, a variety of hydrogel constructs are generated, including those based on poly(ethylene glycol) diacrylate (PEGDA) and gelatin methacryloyl (GelMA). The biocompatibility of this system is validated by introducing cell‐laden GelMA into the microfluidic device and fabricating cellularized constructs. A pattern of a PEGDA frame and three different concentrations of GelMA, loaded with vascular endothelial growth factor, are further assessed for its neovascularization potential in a rat model. The proposed system provides a robust platform for bioprinting of high‐fidelity multimaterial microstructures on demand for applications in tissue engineering, regenerative medicine, and biosensing, which are otherwise not readily achievable at high speed with conventional stereolithographic biofabrication platforms.