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Molecular Cancer Imaging in the Second Near‐Infrared Window Using a Renal‐Excreted NIR‐II Fluorophore‐Peptide Probe
Author(s) -
Wang Weizhi,
Ma Zhuoran,
Zhu Shoujun,
Wan Hao,
Yue Jingying,
Ma Huilong,
Ma Rui,
Yang Qinglai,
Wang Zihua,
Li Qian,
Qian Yixia,
Yue Chunyan,
Wang Yuehua,
Fan Linyang,
Zhong Yeteng,
Zhou Ying,
Gao Hongpeng,
Ruan Junshan,
Hu Zhiyuan,
Liang Yongye,
Dai Hongjie
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201800106
Subject(s) - fluorophore , materials science , peptide , window (computing) , near infrared spectroscopy , infrared , fluorescence , molecular imaging , cancer , optics , nuclear magnetic resonance , medicine , biology , computer science , operating system , physics , microbiology and biotechnology , in vivo
In vivo molecular imaging of tumors targeting a specific cancer cell marker is a promising strategy for cancer diagnosis and imaging guided surgery and therapy. While targeted imaging often relies on antibody‐modified probes, peptides can afford targeting probes with small sizes, high penetrating ability, and rapid excretion. Recently, in vivo fluorescence imaging in the second near‐infrared window (NIR‐II, 1000–1700 nm) shows promise in reaching sub‐centimeter depth with microscale resolution. Here, a novel peptide (named CP) conjugated NIR‐II fluorescent probe is reported for molecular tumor imaging targeting a tumor stem cell biomarker CD133. The click chemistry derived peptide‐dye (CP‐IRT dye) probe afforded efficient in vivo tumor targeting in mice with a high tumor‐to‐normal tissue signal ratio (T/NT > 8). Importantly, the CP‐IRT probes are rapidly renal excreted (≈87% excretion within 6 h), in stark contrast to accumulation in the liver for typical antibody‐dye probes. Further, with NIR‐II emitting CP‐IRT probes, urethra of mice can be imaged fluorescently for the first time noninvasively through intact tissue. The NIR‐II fluorescent, CD133 targeting imaging probes are potentially useful for human use in the clinic for cancer diagnosis and therapy.