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3D Jet Writing: Functional Microtissues Based on Tessellated Scaffold Architectures
Author(s) -
Jordahl Jacob H.,
Solorio Luis,
Sun Hongli,
Ramcharan Stacy,
Teeple Clark B.,
Haley Henry R.,
Lee Kyung Jin,
Eyster Thomas W.,
Luker Gary D.,
Krebsbach Paul H.,
Lahann Joerg
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201707196
Subject(s) - art , art history
The advent of adaptive manufacturing techniques supports the vision of cell‐instructive materials that mimic biological tissues. 3D jet writing, a modified electrospinning process reported herein, yields 3D structures with unprecedented precision and resolution offering customizable pore geometries and scalability to over tens of centimeters. These scaffolds support the 3D expansion and differentiation of human mesenchymal stem cells in vitro. Implantation of these constructs leads to the healing of critical bone defects in vivo without exogenous growth factors. When applied as a metastatic target site in mice, circulating cancer cells home in to the osteogenic environment simulated on 3D jet writing scaffolds, despite implantation in an anatomically abnormal site. Through 3D jet writing, the formation of tessellated microtissues is demonstrated, which serve as a versatile 3D cell culture platform in a range of biomedical applications including regenerative medicine, cancer biology, and stem cell biotechnology.