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Coacervation‐Mediated Combinatorial Synthesis of Biomatrices for Stem Cell Culture and Directed Differentiation
Author(s) -
Wieduwild Robert,
Wetzel Richard,
Husman Dejan,
Bauer Sophie,
ElSayed Iman,
Duin Sarah,
Murawala Priyanka,
Thomas Alvin Kuriakose,
Wobus Manja,
Bornhäuser Martin,
Zhang Yixin
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201706100
Subject(s) - coacervate , extracellular matrix , glycosaminoglycan , materials science , hyaluronic acid , regenerative medicine , mesenchymal stem cell , tissue engineering , cell culture , stem cell , ethylene glycol , stromal cell , cell , biophysics , nanotechnology , microbiology and biotechnology , chemistry , biomedical engineering , biochemistry , biology , anatomy , medicine , genetics , cancer research , organic chemistry
Combinatorial screening represents a promising strategy to discover biomaterials for tailored cell culture applications. Although libraries incorporating different biochemical cues have been investigated, few simultaneously recapitulate relevant biochemical, physical, and dynamic features of the extracellular matrix (ECM). Here, a noncovalent system based on liquid–liquid phase separation (coacervation) and gelation mediated by glycosaminoglycan (GAG)‐peptide interactions is reported. Multiple biomaterial libraries are generated using combinations of sulfated glycosaminoglycans and poly(ethylene glycol)‐conjugated peptides. Screening these biomaterials reveals preferred biomatrices for the attachment of six cell types, including primary mesenchymal stromal cells (MSCs) and primary neural precursor cells (NPCs). Incorporation of GAGs sustains the expansion of all tested cell types comparable to standard cell culture surfaces, while osteogenic differentiation of MSC and neuronal differentiation of NPC are promoted on chondroitin and heparan biomatrices, respectively. The presented noncovalent system provides a powerful tool for developing tissue‐specific ECM mimics.