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3D NIR‐II Molecular Imaging Distinguishes Targeted Organs with High‐Performance NIR‐II Bioconjugates
Author(s) -
Zhu Shoujun,
Herraiz Sonia,
Yue Jingying,
Zhang Mingxi,
Wan Hao,
Yang Qinglai,
Ma Zhuoran,
Wang Yan,
He Jiahuan,
Antaris Alexander L.,
Zhong Yeteng,
Diao Shuo,
Feng Yi,
Zhou Ying,
Yu Kuai,
Hong Guosong,
Liang Yongye,
Hsueh Aaron J.,
Dai Hongjie
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201705799
Subject(s) - molecular imaging , materials science , near infrared spectroscopy , fluorescence lifetime imaging microscopy , fluorescence , bioconjugation , preclinical imaging , microscopy , autofluorescence , nanotechnology , biomedical engineering , biophysics , in vivo , optics , biology , medicine , physics , microbiology and biotechnology
Greatly reduced scattering in the second near‐infrared (NIR‐II) region (1000–1700 nm) opens up many new exciting avenues of bioimaging research, yet NIR‐II fluorescence imaging is mostly implemented by using nontargeted fluorophores or wide‐field imaging setups, limiting the signal‐to‐background ratio and imaging penetration depth due to poor specific binding and out‐of‐focus signals. A newly developed high‐performance NIR‐II bioconjugate enables targeted imaging of a specific organ in the living body with high quality. Combined with a home‐built NIR‐II confocal set‐up, the enhanced imaging technique allows 900 µm‐deep 3D organ imaging without tissue clearing techniques. Bioconjugation of two hormones to nonoverlapping NIR‐II fluorophores facilitates two‐color imaging of different receptors, demonstrating unprecedented multicolor live molecular imaging across the NIR‐II window. This deep tissue imaging of specific receptors in live animals allows development of noninvasive molecular imaging of multifarious models of normal and neoplastic organs in vivo, beyond the traditional visible to NIR‐I range. The developed NIR‐II fluorescence microscopy will become a powerful imaging technique for deep tissue imaging without any physical sectioning or clearing treatment of the tissue.