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Biofunctionalized “Kiwifruit‐Assembly” of Oxidoreductases in Mesoporous ZnO/Carbon Nanoparticles for Efficient Asymmetric Catalysis
Author(s) -
Zhang Rongzhen,
Jiang Jiawei,
Zhou Junping,
Xu Yan,
Xiao Rong,
Xia Xinhui,
Rao Zhiming
Publication year - 2018
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201705443
Subject(s) - catalysis , yield (engineering) , mesoporous material , materials science , substrate (aquarium) , nanoparticle , combinatorial chemistry , electron transfer , chemical engineering , nanotechnology , organic chemistry , chemistry , engineering , metallurgy , oceanography , geology
A mesoporous ZnO/carbon composite is designed for coimmobilization of two oxidoreductases involving a novel “kiwifruit‐assembly” pattern. The coimmobilization of ( S )‐carbonyl reductase II‐glucose dehydrogenase on nanoparticles (SCRII–GDH nano ) exhibits 40–50% higher specific activity than the free enzyme and significantly improves stabilities of enzymes to heat, pH and solvents. It performs asymmetric catalysis of 75 × 10 −3 m substrate with a perfect yield of 100% and an excellent enantioselectivity of 99.9% within 1 h. SCRII–GDH nano gives an over 72% yield and 99.9% enantioselectivity after it is reused for ten times. Even with a highly concentrated (400 × 10 −3 m ) substrate, it shows about 60% yield and 99.9% enantioselectivity within 4 h. SCRII–GDH nano presents 4.5–8.0‐fold higher productivity in 2.0–8.0‐fold shorter reaction time than the free enzyme. This work provides a general, facile, and unique approach for the immobilization of two oxidoreductases and gives high catalytic efficiency, long‐term and good recycling stabilities by triggering radical proton‐coupled electron transfer.