Premium
Two‐Photon In Vivo Imaging with Porous Silicon Nanoparticles
Author(s) -
Kim Dokyoung,
Kang Jinyoung,
Wang Taejun,
Ryu Hye Gun,
Zuidema Jonathan M.,
Joo Jinmyoung,
Kim Muwoong,
Huh Youngbuhm,
Jung Junyang,
Ahn Kyo Han,
Kim Ki Hean,
Sailor Michael J.
Publication year - 2017
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201703309
Subject(s) - materials science , autofluorescence , photoluminescence , porous silicon , nanoparticle , optoelectronics , luminescence , light scattering , photon , scattering , absorption (acoustics) , wavelength , quantum yield , fluorescence , silicon , quantum dot , two photon excitation microscopy , near infrared spectroscopy , nanotechnology , optics , physics , composite material
A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near‐infrared (NIR) emitters that can also be excited with NIR radiation via two‐photon processes can mitigate these factors somewhat because they operate at wavelengths of 650–1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two‐photon‐absorption cross‐section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.