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Precise and Arbitrary Deposition of Biomolecules onto Biomimetic Fibrous Matrices for Spatially Controlled Cell Distribution and Functions
Author(s) -
Jia Chao,
Luo Bowen,
Wang Haoyu,
Bian Yongqian,
Li Xueyong,
Li Shaohua,
Wang Hongjun
Publication year - 2017
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201701154
Subject(s) - biomolecule , materials science , extracellular matrix , micropatterning , nanotechnology , stromal cell , tissue engineering , nanofiber , electrospinning , nanomedicine , cancer cell , angiogenesis , biomedical engineering , microbiology and biotechnology , cancer , biology , polymer , nanoparticle , medicine , genetics , cancer research , composite material
Advances in nano‐/microfabrication allow the fabrication of biomimetic substrates for various biomedical applications. In particular, it would be beneficial to control the distribution of cells and relevant biomolecules on an extracellular matrix (ECM)‐like substrate with arbitrary micropatterns. In this regard, the possibilities of patterning biomolecules and cells on nanofibrous matrices are explored here by combining inkjet printing and electrospinning. Upon investigation of key parameters for patterning accuracy and reproducibility, three independent studies are performed to demonstrate the potential of this platform for: i) transforming growth factor (TGF)‐β1‐induced spatial differentiation of fibroblasts, ii) spatiotemporal interactions between breast cancer cells and stromal cells, and iii) cancer‐regulated angiogenesis. The results show that TGF‐β1 induces local fibroblast‐to‐myofibroblast differentiation in a dose‐dependent fashion, and breast cancer clusters recruit activated stromal cells and guide the sprouting of endothelial cells in a spatially resolved manner. The established platform not only provides strategies to fabricate ECM‐like interfaces for medical devices, but also offers the capability of spatially controlling cell organization for fundamental studies, and for high‐throughput screening of various biomolecules for stem cell differentiation and cancer therapeutics.