Premium
Antibiotic Spider Silk: Site‐Specific Functionalization of Recombinant Spider Silk Using “Click” Chemistry
Author(s) -
Harvey David,
Bardelang Philip,
Goodacre Sara L.,
Cockayne Alan,
Thomas Neil R.
Publication year - 2017
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201604245
Subject(s) - silk , click chemistry , spider silk , polymer science , spider , chemistry , nanotechnology , computer science , combinatorial chemistry , materials science , biology , ecology , operating system
In a new, versatile approach to functionalizing recombinant spider silk , L‐azidohomoalanine is introduced residue‐specifically in the minispidroin protein 4RepCT through expression in an E. coli methionine auxotroph. Both fluorophores and the antibiotic levofloxacin are attached to this bio‐orthogonal amino acid using copper‐catalyzed click chemistry, either before or after the silk fibers are self‐assembled.