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Light‐Patterned RNA Interference of 3D‐Cultured Human Embryonic Stem Cells
Author(s) -
Huang Xiao,
Hu Qirui,
Lai Yifan,
Morales Demosthenes P.,
Clegg Dennis O.,
Reich Norbert O.
Publication year - 2016
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201603318
Subject(s) - embryonic stem cell , materials science , rna , downregulation and upregulation , stem cell , rna interference , gene , nanotechnology , microbiology and biotechnology , computational biology , optoelectronics , biology , genetics
A new method of spatially controlled gene regulation in 3D‐cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near‐infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two‐photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.