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Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles
Author(s) -
Chiappini Ciro,
Campagnolo Paola,
Almeida Carina S.,
AbbassiGhadi Nima,
Chow Lesley W.,
Hanna George B.,
Stevens Molly M.
Publication year - 2015
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201501304
Subject(s) - materials science , cathepsin b , cathepsin , porous silicon , cathepsin d , esophageal cancer , silicon , nanotechnology , cancer , biophysics , enzyme , optoelectronics , biochemistry , biology , medicine
Porous silicon nanoneedles can map Cathepsin B activity across normal and tumor human esophageal mucosa. Assembling a peptide‐based Cathepsin B cleavable sensor over a large array of nanoneedles allows the discrimination of cancer cells from healthy ones in mixed culture. The same sensor applied to tissue can map Cathepsin B activity with high resolution across the tumor margin area of esophageal adenocarcinoma.