Premium
DhITACT: DNA Hydrogel Formation by Isothermal Amplification of Complementary Target in Fluidic Channels
Author(s) -
Lee Ho Yeon,
Jeong Hansaem,
Jung Il Young,
Jang Bora,
Seo Young Chang,
Lee Haeshin,
Lee Hyukjin
Publication year - 2015
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201500414
Subject(s) - loop mediated isothermal amplification , microfluidics , fluidics , dna , isothermal process , materials science , nanotechnology , matching (statistics) , computer science , biology , genetics , physics , engineering , electrical engineering , thermodynamics , statistics , mathematics
DNA hydrogel formation by isothermal amplification of complementary targets in microfluidic channels (DhITACT) is a new platform for rapid and accurate detection of infectious pathogens. DNA hydrogel is formed in situ within microfluidic channels by the isothermal rolling circle amplification process upon the selective binding of target strands from the biological fluid. Once the volume of DNA hydrogel sufficiently enlarges, it can selectively block the matching channels with target pathogens.