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Transparent, Nanoporous, and Transferable Membrane‐Based Cell–Cell Paracrine Signaling Assay
Author(s) -
Jang Yeongseon,
Lee Hyojin,
Char Kookheon,
Nam JwaMin
Publication year - 2015
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201404863
Subject(s) - paracrine signalling , stromal cell , microbiology and biotechnology , tumor microenvironment , cancer cell , invadopodia , biology , cell signaling , cell type , cell , cancer research , signal transduction , immune system , immunology , cancer , biochemistry , receptor , genetics
A transparent, nanoporous, and transferable membrane‐based cell coculture platform is devised and developed to be used for the accurate and versatile control and analysis of paracrine communications between cocultured cells, as well as easy isolation of each cell line for further manipulation and analysis. On the basis of the high degree of freedom in stacking and destacking of the membranes and the controllable nanopores that efficiently allow exchanges of paracrine factors without physical contact between the different cell lines, the paracrine signaling between human breast metastatic cancer cells and three different types of stromal cell types are controlled and studied.