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Sensors: Real‐Time, Sensitive, and Specific Detection of Promoter‐Polymerase Interactions in Gene Transcription Using a Nanoplasmonic Sensor (Adv. Mater. 9/2013)
Author(s) -
Song Min Sun,
Choi Seung Phill,
Lee Jeewon,
Kwon Young Jik,
Sim Sang Jun
Publication year - 2013
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201370053
Subject(s) - surface plasmon resonance , materials science , rna polymerase , colloidal gold , polymerase , microbiology and biotechnology , transcription (linguistics) , biophysics , nanotechnology , nanoparticle , rna , gene , biology , biochemistry , linguistics , philosophy
Sang Jun Sim and co‐workers demonstrate on page 1265 the specific recognition of pointmutated promoter by RNA polymerase. The interactions between promoter and RNA polymerase are monitored using Rayleigh light scattering on gold nanoparticles. Using a darkfield microscope, the individual gold nanoparticles can be visualized as the bright colored spots (red, green, or orange in the cover image) by light scattering. Binding of RNA polymerase on the surface of a AuNP is quantitatively detected by LSPR (localized surface plasmon resonance) maximum wavelength shift.