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Substrates with Patterned Extracellular Matrix and Subcellular Stiffness Gradients Reveal Local Biomechanical Responses
Author(s) -
Tseng Peter,
Di Carlo Dino
Publication year - 2014
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201304607
Subject(s) - fabrication , extracellular matrix , nanotechnology , materials science , photolithography , rigidity (electromagnetism) , computer science , biology , microbiology and biotechnology , composite material , medicine , alternative medicine , pathology
A substrate fabrication process is developed to pattern both the extracellular matrix (ECM) and rigidity at sub‐cellular spatial resolution. When growing cells on these substrates, it is found that cells respond locally in their cytoskeleton assembly. The presented method allows unique insight into the biological interpretation of mechanical signals, whereas photolithography‐based fabrication is amenable to integration with complex microfabricated substructures.