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Viral Mimicking Ternary Polyplexes: A Reduction‐Controlled Hierarchical Unpacking Vector for Gene Delivery
Author(s) -
He Yiyan,
Nie Yu,
Cheng Gang,
Xie Li,
Shen Youqing,
Gu Zhongwei
Publication year - 2014
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201304592
Subject(s) - diselenide , materials science , gene delivery , conjugated system , ethylene glycol , transfection , dna , unpacking , in vivo , imine , biophysics , in vitro , cleavage (geology) , gene , biochemistry , chemistry , biology , organic chemistry , polymer , selenium , catalysis , genetics , linguistics , philosophy , fracture (geology) , metallurgy , composite material
Reduction‐controlled hierarchical unpacking is proposed for the development of virus‐mimicking gene carriers. Disulfide‐bond‐modified hyaluronic acid (HA) is deposited onto the surface of diselenide‐conjugated oligoethylenimine/DNA polyplexes to form DNA/OEI‐SeSe x /HA‐SS‐COOH (DOS) polyplexes. The cleavage of the disulfide and diselenide bonds is triggered by the gradient GSH level at the tumor site and inside the cells. The transfection efficiency of DOS show significant enhancement over DNA/poly(ethylene imine) (DP) in vitro and in vivo.