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Anti tags: SERS‐Encoded Nanoparticle Assemblies that Enable Single‐Spot Multiplex Protein Detection
Author(s) -
Guarrotxeekane,
Bazan Guillermo C.
Publication year - 2014
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201304107
Subject(s) - multiplex , materials science , nanotechnology , ethylene glycol , multiplexing , nanoparticle , surface enhanced raman spectroscopy , peg ratio , raman spectroscopy , raman scattering , computer science , chemistry , optics , bioinformatics , biology , telecommunications , physics , organic chemistry , finance , economics
Simultaneous detection of multiple proteins on a single spot can be efficiently achieved by using multiplexed surface‐enhanced Raman spectroscopy (SERS)‐encoded nanoparticle ‘antitags’ consisting of poly(ethylene glycol) (PEG)‐protected silver dimers (and higher aggregates) and antibody‐tagging entities. The effective SERS‐based multivariate deconvolution approach guarantees an accurate and successful distinguishable identification of single and multiple proteins in complex samples. Their potential application in multiplexed SERS bioimaging technology can be easily envisaged.