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Triggered Sorting and Co‐Assembly of Genetically Engineered Protein Microdomains in the Cytoplasm
Author(s) -
Shi Pu,
Lin YiAn,
Pastuszka Martha,
Cui Honggang,
MacKay J. Andrew
Publication year - 2014
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201303356
Subject(s) - pharmaceutical sciences , library science , chemistry , medicine , pharmacology , computer science
Elastin‐like polypeptides (ELPs) are genetically encoded protein polymers that reversibly phase separate in response to stimuli. They respond sharply to small shifts in temperature and form dense microdomains in the living eukaryotic cytosol. For the first time, this communication illustrates how to tune the ELP sequence and architecture for either co‐assembly or sorting of distinct proteins into microdomains within a living cell.