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Complex Stiffness Gradient Substrates for Studying Mechanotactic Cell Migration (Adv. Mater. 45/2012)
Author(s) -
Kuo ChengHwa R.,
Xian Jian,
Brenton James D.,
Franze Kristian,
Sivaniah Easan
Publication year - 2012
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201290273
Subject(s) - materials science , stiffness , composite material , layer (electronics)
Complex stiffness gradient substrates for the study of mechanotactic cell migration are developed by Easan Sivaniah, Kristian Franze, and co‐workers on page 6059 . Polyacrylamide gels are cast upon a stiff support with controlled topography, resulting in a thin gel layer of variable height. The topographical profiles project a stiffness map onto the gel, resulting in controlled linear and non‐linear 2D stiffness gradients. Fibroblasts, which migrate towards stiffer substrates, accumulate in areas with a gel thickness below 15 μm.