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Purification of High‐Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold‐Coated Membranes
Author(s) -
Schirwitz Christopher,
Loeffler Felix F.,
Felgenhauer Thomas,
Stadler Volker,
NesterovMueller Alexander,
Dahint Reiner,
Breitling Frank,
Bischoff F. Ralf
Publication year - 2013
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201203853
Subject(s) - peptide , membrane , covalent bond , materials science , cysteine , dna microarray , protein array analysis , peptide library , combinatorial chemistry , nanotechnology , biophysics , peptide sequence , biochemistry , chemistry , biology , enzyme , organic chemistry , gene expression , gene
A method for the one‐step purification of high‐complexity peptide microarrays is presented . The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full‐length peptides covalently couple to the receptor membrane via an N‐terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm 2 is demonstrated.