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A Sensitive H 2 O 2 Assay Based on Dumbbell‐like PtPd‐Fe 3 O 4 Nanoparticles
Author(s) -
Sun Xiaolian,
Guo Shaojun,
Chung ChunShiang,
Zhu Wenlei,
Sun Shouheng
Publication year - 2013
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201203218
Subject(s) - nanoparticle , detection limit , dumbbell , materials science , nanotechnology , oleylamine , combinatorial chemistry , computer science , chemistry , chromatography , biology , physiology
Dumbbell‐like Pt 48 Pd 52 ‐Fe 3 O 4 nanoparticles are synthesized and functionalized with oleylamine‐polyethyleneglycol to serve as an efficient catalyst for H 2 O 2 reduction and tetramethylbenzidine (TMB) oxidation in biological solutions. The Pt 48 Pd 52 ‐Fe 3 O 4 /TMB kit is even more active than the natural enzyme for H 2 O 2 detection with a detection limit reaching 2 μM, and is successfully used to quantitatively monitor the extracellular H 2 O 2 generated by neutrophils.