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Label‐Free Ultrasensitive Detection of Human Telomerase Activity Using Porphyrin‐Functionalized Graphene and Electrochemiluminescence Technique
Author(s) -
Wu Li,
Wang Jiasi,
Feng Lingyan,
Ren Jinsong,
Wei Weili,
Qu Xiaogang
Publication year - 2012
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201200412
Subject(s) - telomerase , detection limit , hela , porphyrin , graphene , electrochemiluminescence , materials science , nanotechnology , dna , microbiology and biotechnology , combinatorial chemistry , chromatography , cell , biochemistry , biology , chemistry , gene
Using porphyrin‐functionalized graphene , we construct a PCR‐free, low‐cost, rapid, and electrochemiluminenscence (ECL) assay for detection of telomerase activity that has been demonstrated in six different cell lines and can be used as initial screening of G‐quadruplex DNA binding agents and telomerase inhibitors. This ECL sensor shows highly sensitive for detection of telomerase with the detection limit as low as 10 HeLa cells mL −1 .