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Coloured Semi‐reflective Thin Films for Biomass‐hydrolyzing Enzyme Detection
Author(s) -
Cerclier Carole,
GuyomardLack Aurélie,
Moreau Céline,
Cousin Fabrice,
Beury Nadège,
Bonnin Estelle,
Jean Bruno,
Cathala Bernard
Publication year - 2011
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201101971
Subject(s) - xyloglucan , materials science , thin film , naked eye , cellulose , characterization (materials science) , deposition (geology) , enzymatic hydrolysis , hydrolysis , enzyme , layer (electronics) , nanotechnology , chemical engineering , optoelectronics , chromatography , organic chemistry , detection limit , chemistry , sediment , paleontology , biology , engineering
A new enzymatic activity detection assay based on colour change of the semi‐reflective films is presented. The method is based on the preparation of multilayered thin films of controlled thickness obtained by sequential deposition of cellulose nanocrystals and xyloglucan. The hydrolysis of the films leads to a decrease in layer thickness that enables to detect enzyme activity, to the naked eye, from the resulting colour changes in a span of few minutes. The method allows direct, fast, highly sensitive, and easy‐to‐use characterization of enzymatic activities.