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Microgels for Efficient Protein Purification
Author(s) -
Mizrahi Boaz,
Irusta Silvia,
McKenna Marshall,
Stefanescu Cristina,
Yedidsion Liron,
Myint MyatNoeZin,
Langer Robert,
Kohane Daniel S.
Publication year - 2011
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201101258
Subject(s) - materials science , ligand (biochemistry) , biomolecule , nanoparticle , protein purification , chelation , lysis , matrix (chemical analysis) , combinatorial chemistry , mcherry , chromatography , nanotechnology , chemistry , biochemistry , receptor , green fluorescent protein , metallurgy , gene
Microgel particles with pore structure and ligands distributed evenly throughout their matrices overcome the major limitations of protein purification systems: low ligand density on the immobilized matrix and protein access to those ligands. A straightforward synthetic scheme for a highly efficient microgel matrix is reported.