Premium
Direct Electrical Measurement of Protein–Water Interactions and Temperature Dependence Using Piezoelectric Microcantilevers
Author(s) -
Lee Jeong Hoon,
Hwang Kyo Seon,
Yoon Dae Sung,
Kang Ji Yoon,
Kim Sang Kyung,
Kim Tae Song
Publication year - 2011
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.201101037
Subject(s) - materials science , monoclonal antibody , piezoelectricity , humidity , protein–protein interaction , biophysics , chemical physics , biological system , antibody , thermodynamics , composite material , biology , chemistry , microbiology and biotechnology , physics , immunology
A platform that provides a fundamental tool to understand protein behaviors by a simple real‐time electrical readout is demonstrated. Observing the temperature dependence of protein activity as well as the humidity‐sensitive protein activity of a prostate‐specific antigen (PSA) monoclonal antibody receptor (MAbs), as well as PSA–MAbs receptor complexes, provides a stability curve for the protein and a humidity dependence of their activity.