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Simple Fabrication of Antibody Microarrays on Nonfouling Polymer Brushes with Femtomolar Sensitivity for Protein Analytes in Serum and Blood
Author(s) -
Hucknall Angus,
Kim DongHwan,
Rangarajan Srinath,
Hill Ryan T.,
Reichert William M.,
Chilkoti Ashutosh
Publication year - 2009
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200803125
Subject(s) - atom transfer radical polymerization , analyte , ethylene glycol , methacrylate , polymer , polymer brush , materials science , peg ratio , combinatorial chemistry , polymerization , polymer chemistry , nanotechnology , chemistry , chromatography , organic chemistry , finance , economics , composite material
A multianalyte antibody array that is spotted on a poly(oligo(ethylene glycol) methacrylate) brush 100 nm thick, grown on glass via surface‐initiated atom transfer radical polymerization, has femtomolar limit‐of‐detection (LOD) of cytokines in serum and whole blood, and a dynamic range of six orders of magnitude for a range of protein analytes.