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A Novel Approach to Observing Synergy Effects of PHSRN on Integrin–RGD Binding Using Intelligent Surfaces
Author(s) -
Ebara Mitsuhiro,
Yamato Masayuki,
Aoyagi Takao,
Kikuchi Akihiko,
Sakai Kiyotaka,
Okano Teruo
Publication year - 2008
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200702308
Subject(s) - lower critical solution temperature , materials science , integrin , peptide , ligand (biochemistry) , shielded cable , polymer , biophysics , receptor , combinatorial chemistry , nanotechnology , composite material , biochemistry , copolymer , chemistry , computer science , biology , telecommunications
A novel assay for measuring time‐dependant ligand‐receptor affinity changes is developed based on a peptide‐immobilized temperature‐responsive surface, as schematically illustrated in the figure. The grafted thermoresponsive polymer acts as an “on‐off” switch for mediating integrin–peptide bonding. At temperatures above and below the lower critical solution temperature (LCST), the peptides are accessible and shielded from integrin access, respectively.