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Quantifying the Performance of Protein‐Resisting Surfaces at Ultra‐Low Protein Coverages using Kinesin Motor Proteins as Probes
Author(s) -
Katira P.,
Agarwal A.,
Fischer T.,
Chen H.Y.,
Jiang X.,
Lahann J.,
Hess H.
Publication year - 2007
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200701982
Subject(s) - kinesin , nanobiotechnology , materials science , motor protein , protein adsorption , microtubule , nanotechnology , characterization (materials science) , fouling , kinetics , biophysics , chemistry , nanoparticle , biochemistry , membrane , polymer , microbiology and biotechnology , biology , composite material , physics , quantum mechanics
The binding kinetics of fluorescently labeled microtubules to kinesin‐coated surfaces permits the determination of the density of kinesin motor proteins adhered to the surface in the range of 0.1 – 30 μm –2 . This extreme sensitivity, corresponding to protein coverages of 0.004 – 1 ng cm –2 , enables the characterization of advanced non‐fouling coatings, such as (EG) 3 OH‐terminated SAMs and PEGMA with applications in biomedical engineering and bionanotechnology.