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Tissue Engineering Based on Cell Sheet Technology
Author(s) -
Matsuda N.,
Shimizu T.,
Yamato M.,
Okano T.
Publication year - 2007
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200701978
Subject(s) - tissue engineering , materials science , adhesion , cell adhesion , cell , monolayer , regeneration (biology) , biomedical engineering , cell culture , nanotechnology , microbiology and biotechnology , composite material , chemistry , biology , medicine , biochemistry , genetics
Abstract Cell sheet technology enables novel approaches to tissue engineering without the use of biodegradable scaffolds. Cell sheet technology consists of a temperature‐responsive culture dish, which enables reversible cell adhesion to and detachment from the dish surface by controllable hydrophobicity of the surface. This allows for a non‐invasive harvest of cultured cells as an intact monolayer cell sheet including deposited extra cellular matrices. The monolayer cell sheet can be transplanted to host tissues without using biodegradable scaffolds and sutures. Thick tissue constructs and patterned cell sheets using two or more kinds of cell source are also developed by means of layered cell sheets in vitro. This Progress Report summarizes temperature‐controlled cell adhesion‐detachment behavior and applications of the cell sheet technology to regeneration of cornea, periodontal ligament, bladder epithelia, oesophageal epithelia, myocardium, and liver.