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Enzyme‐Based Multi‐Component Optical Nanoprobes for Sequence‐ Specific Detection of DNA Hybridization
Author(s) -
Li J.,
Song S.,
Liu X.,
Wang L.,
Pan D.,
Huang Q.,
Zhao Y.,
Fan C.
Publication year - 2008
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200701918
Subject(s) - nanoprobe , bovine serum albumin , horseradish peroxidase , dna , nanoparticle , biosensor , nanotechnology , component (thermodynamics) , materials science , combinatorial chemistry , enzyme , microbiology and biotechnology , computational biology , biochemistry , chemistry , biology , physics , thermodynamics
Seeing is believing. A novel multi‐ component gold nanoparticle‐based nanoprobe is assembled with a thiolated DNA detection probe, horseradish peroxidase (HRP), and bovine serum albumin (BSA). In this nanoprobe, the DNA detection probe is used to construct complexes for “sandwich”‐based DNA detection, HRP translates this hybridization event into an enzymatic catalysis‐based optical signal, while and BSA acts as a non‐specific blocker.