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Synthesis of Functionalized Au Nanoparticles for Protein Detection
Author(s) -
Ja. R.,
Ying J. Y.
Publication year - 2008
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200701348
Subject(s) - materials science , protein detection , nanoparticle , aptamer , nanotechnology , naked eye , western blot , combinatorial chemistry , biophysics , chromatography , microbiology and biotechnology , detection limit , chemistry , biochemistry , biology , gene
Aptamer and antibody functionalized Au nanoparticles are synthesized and used for protein detection (see figure). These particles are highly water soluble and as small as 10 nm. However, they are enlarged after a protein binding event to enhance signal sensitivity. A conventional western blot protocol is used to enable detection of the proteins with nanomolar sensitivity, with the naked eye.