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Multilayer Nanocomplexes of Polymer and DNA Exhibit Enhanced Gene Delivery
Author(s) -
Saul J. M.,
Wang C.H. K.,
Ng C. P.,
Pun S. H.
Publication year - 2008
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200700633
Subject(s) - polyethylenimine , gene delivery , transgene , dna , polymer , reporter gene , materials science , gene , genetic enhancement , plasmid , microbiology and biotechnology , nanotechnology , gene expression , biophysics , transfection , biology , biochemistry , composite material
Polymeric‐DNA complexes (polyplexes) are constructed with multiple layers of counter‐polyions as DNA/polyethylenimine/poly(acrylic acid)/polyethylenimine. The increased association of polyethylenimine achieved by the multilayer approach leads to substantial increases in expression of transgene for reporter plasmids without the need for excess free polymer typically required for non‐viral gene delivery. This method of polyplex preparation provides the opportunity to improve transgene expression for gene therapy approaches to disease treatment.