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Cationic Conjugated Polymer/DNA Complexes for Amplified Fluorescence Assays of Nucleases and Methyltransferases
Author(s) -
Feng F.,
Tang Y.,
He F.,
Yu M.,
Duan X.,
Wang S.,
Li Y.,
Zhu D.
Publication year - 2007
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200700165
Subject(s) - conjugated system , methyltransferase , cationic polymerization , fluorescence , dna , materials science , polymer , nanotechnology , genetics , biology , polymer chemistry , methylation , optics , physics , composite material
A new method is developed for sensitive, homogeneous, and convenient assays of nucleases and methyltransferases using complexes of cationic conjugated polymers with DNA containing a fluorescein tag at the 5′‐terminus (DNA‐Fl). The cleavage of DNA by nucleases can be monitored by fluorescence spectra by observing conjugated‐polymer or fluorescein emission changes in aqueous solutions.