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Myogenic Induction of Aligned Mesenchymal Stem Cell Sheets by Culture on Thermally Responsive Electrospun Nanofibers
Author(s) -
Dang J. M.,
Leong K. W.
Publication year - 2007
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200602159
Subject(s) - materials science , mesenchymal stem cell , nanofiber , cytoskeleton , stem cell , tissue engineering , microbiology and biotechnology , substrate (aquarium) , cellular differentiation , elongation , biophysics , regenerative medicine , nanotechnology , cell , biomedical engineering , biology , composite material , ultimate tensile strength , biochemistry , medicine , ecology , gene
A thermally reversible culture substrate with a topographically active surface of aligned nanofibers is able to induce cytoskeletal alignment and nucleus elongation. These morphological changes induce myogenic differentiation in stem cells. The differentiated cells can be recovered in sheet form by thermally induced dissolution of the substrate. A surface able to provide topographical cues to create an aligned stem cell sheet, activated to differentiate to a specific lineage, can have a significant impact on engineering of tissue constructs for regenerative medicine applications.