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Fabrication of Oligonucleotide Chips by Using Parallel Cantilever‐Based Electrochemical Deposition in Picoliter Volumes
Author(s) -
Descamps E.,
Leïchlé T.,
Corso B.,
Laurent S.,
Mailley P.,
Nicu L.,
Livache T.,
Bergaud C.
Publication year - 2007
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200602152
Subject(s) - materials science , biochip , polypyrrole , cantilever , fabrication , deposition (geology) , nanotechnology , oligonucleotide , microfabrication , electrochemistry , micrometer , reproducibility , polymer , electrode , composite material , polymerization , chromatography , chemistry , alternative medicine , pathology , biology , dna , paleontology , medicine , sediment , optics , biochemistry , physics
The use of a parallel cantilever‐based deposition system as an electrospotting tool in picoliter volumes is demonstrated. Applied in conjunction with polypyrrole electrochemistry, this generic approach for fabricating biochips is optimized for oligonucleotide chips featuring micrometer spots of different sequences with high reproducibility and specificity (see figure).