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Two‐Photon Excitation of Quantum‐Dot‐Based Fluorescence Resonance Energy Transfer and Its Applications
Author(s) -
Clapp A. R.,
Pons T.,
Medintz I. L.,
Delehanty J. B.,
Melinger J. S.,
Tiefenbrunn T.,
Dawson P. E.,
Fisher B. R.,
O'Rourke B.,
Mattoussi H.
Publication year - 2007
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200602036
Subject(s) - förster resonance energy transfer , quantum dot , excitation , fluorescence , excited state , materials science , resonance (particle physics) , absorption (acoustics) , luminescence , resonant inductive coupling , photon , optoelectronics , energy transfer , photochemistry , molecular physics , atomic physics , physics , chemistry , optics , quantum mechanics , composite material
Nonradiative fluorescence resonance energy transfer (FRET) between a luminescent quantum dot (QD) donor and a proximal dye brought in close proximity of the QD surface via conjugation with a dye‐labeled peptide (or a protein) is shown (see figure). The system is excited with near IR irradiation (well below the absorption band of the QD), via a fast two‐photon process, which produces a FRET signal with very low background contribution due to a substantially reduced nonlinear direct excitation of the dye.