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Synthesis of Magnetic Microspheres with Immobilized Metal Ions for Enrichment and Direct Determination of Phosphopeptides by Matrix‐Assisted Laser Desorption Ionization Mass Spectrometry
Author(s) -
Xu X.,
Deng C.,
Gao M.,
Yu W.,
Yang P.,
Zhang X.
Publication year - 2006
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200601546
Subject(s) - phosphopeptide , microsphere , mass spectrometry , materials science , desorption , matrix assisted laser desorption/ionization , metal , matrix (chemical analysis) , analytical chemistry (journal) , ion , ionization , nanotechnology , chromatography , chemical engineering , chemistry , organic chemistry , adsorption , biochemistry , kinase , engineering , metallurgy , composite material
Core/shell structured magnetic silica microspheres with immobilized Fe 3+ (see image) are prepared with a simple method. Using a magnetic field, the microspheres are quickly, efficiently, and specifically enriched. Phosphopeptide identification can then be achieved through mass spectroscopy. This is a new method for the enrichment of phosphopeptides and opens up the possibility of other new applications using the microspheres.