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Protein‐Coated Porous‐Silicon Photonic Crystals for Amplified Optical Detection of Protease Activity
Author(s) -
Orosco M. M.,
Pacholski C.,
Miskelly G. M.,
Sailor M. J.
Publication year - 2006
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200502420
Subject(s) - protease , materials science , biosensor , cleavage (geology) , photonic crystal , layer (electronics) , photonics , porous medium , porosity , nanotechnology , optoelectronics , enzyme , chemistry , biochemistry , composite material , fracture (geology)
An optical biosensor for protease activity , capable of detecting picomole quantities of protease, is constructed from a 1D photonic crystal of porous Si. The porous Si layer is coated with a thin film of zein, a natural hydrophobic protein. Proteolytic cleavage produces small fragments that infiltrate the pores of the photonic crystal, producing a distinct color change (see figure). The amount of protease pepsin used on each of the spots is indicated.