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Conjugated‐Polymer/DNA Interpolyelectrolyte Complexes for Accurate DNA Concentration Determination
Author(s) -
Hong J. W.,
Hemme W. L.,
Keller G. E.,
Rinke M. T.,
Bazan G. C.
Publication year - 2006
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200501605
Subject(s) - conjugated system , dna , cationic polymerization , intercalation (chemistry) , materials science , polymer , polyelectrolyte , fluorescence , quenching (fluorescence) , electrolyte , photochemistry , polymer chemistry , chemistry , organic chemistry , optics , biochemistry , physics , electrode , composite material
Precise determination of DNA concentration without the health risks of intercalating dyes is possible via the optical changes that arise from DNA‐induced aggregation of cationic conjugated polyelectrolytes. Addition of DNA to a conjugated electrolyte containing two fluorophores results in aggregation and fluorescence quenching, inducing a measurable and quantifiable shift from blue‐ to green‐light emission (see figure and inside cover).