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Biologically Active Protein Gradients via Microstamping
Author(s) -
Bhangale S. M.,
Tjong V.,
Wu L.,
Yakovlev N.,
Moran P. M.
Publication year - 2005
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200400547
Subject(s) - materials science , polymer , substrate (aquarium) , silicon , nanotechnology , chemical engineering , optoelectronics , composite material , biology , ecology , engineering
Protein gradients on substrates are prepared using a microstamping technique. An Au pattern is transferred from a patterned silicon stamp to a polymeric substrate, and exposed polymer regions are then selectively activated for protein immobilization. The protein gradient is achieved by varying the spacing between the exposed polymer regions (see Figure).