Premium
Flow‐Through‐Type DNA Array Based on Ideally Ordered Anodic Porous Alumina Substrate
Author(s) -
Matsumoto F.,
Nishio K.,
Masuda H.
Publication year - 2004
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200400360
Subject(s) - materials science , substrate (aquarium) , porosity , fluorescence , fabrication , anode , nanotechnology , dna , micrometer , fluorescence microscope , electrode , composite material , optics , chemistry , medicine , biochemistry , oceanography , physics , alternative medicine , pathology , geology
A flow‐through‐type DNA array fabricated from an ideally ordered anodic porous alumina substrate is reported. Single‐stranded probe DNA was affixed to the walls of the sub‐micrometer‐diameter holes, and fluorescently labelled target DNA was passed through the holes in the substrate. Fluorescence microscopy revealed an ordered array of fluorescent spots after the hybridization (see Figure), demonstrating the applicability of these porous alumina substrates to fabrication of high‐density DNA arrays.