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Size‐Controlled Formation of a Calcium Phosphate‐Based Organic–Inorganic Hybrid Vector for Gene Delivery Using Poly(ethylene glycol)‐ block ‐poly(aspartic acid)
Author(s) -
Kakizawa Y.,
Miyata K.,
Furukawa S.,
Kataoka K.
Publication year - 2004
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200305782
Subject(s) - ethylene glycol , materials science , nuclease , copolymer , calcium , gene delivery , peg ratio , phosphate , aspartic acid , chemical engineering , dna , polymer chemistry , polymer , transfection , gene , biochemistry , chemistry , amino acid , finance , economics , engineering , metallurgy , composite material
Poly(ethylene glycol) (PEG)‐covered hybrid particles (see Figure) of calcium phosphate (CaP), and plasmid DNA (pDNA) have been prepared in a size‐controllable manner in the presence of a block copolymer. The particles show a substantial colloidal stability as well as tolerance of entrapped pDNA against nuclease attack in the medium, and achieve a significantly higher gene expression compared to conventional precipitates.