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A Novel Hierarchical Nanozeolite Composite as Sorbent for Protein Separation in Immobilized Metal‐Ion Affinity Chromatography
Author(s) -
Xu F.,
Wang Y.,
Wang X.,
Zhang Y.,
Tang Y.,
Yang P.
Publication year - 2003
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200305287
Subject(s) - sorbent , materials science , composite number , chromatography , affinity chromatography , ion exchange , zeolite , ion chromatography , metal , metal ions in aqueous solution , histidine , chemical engineering , ion , organic chemistry , chemistry , adsorption , catalysis , composite material , metallurgy , enzyme , engineering
A new hierarchical porous zeolite–diatomite composite (see Figure inset) of excellent mechanical and chemical stability, after immobilization of Co 2+ by an ion‐exchange process, was utilized as a packing sorbent for the separation of peptides and proteins containing histidine clusters. The Figure shows the chromatographic profile for two peptides separated using immobilized metal‐ion affinity chromatography.